Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: ( a ) Western blot analysis of EGFR, Nectin-4, and TROP-2 expression in the BC cell lines RT4 and RT112 and in the antigen-negative control cell line CHO. ( b ) Cell binding of the antibodies and conjugates to the target cells as demonstrated by flow cytometry. Binding of the isotype control antibody is shown in gray.

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Western Blot, Expressing, Negative Control, Binding Assay, Flow Cytometry, Control

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2, and Sac T120C/D265C -WB692-CB2 after irradiation with different light doses in RT4 and RT112 cells. CHO cells served as antigen-negative controls. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Irradiation

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2 and Sac 120T/265C -WB692-CB2 alone and in combination after irradiation with a light dose of 64 J/cm 2 in RT4 and RT112 cells. Control samples were treated with the uncoupled antibodies or the free dye. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Irradiation, Control

Journal: Oncogenesis

Article Title: Adaptation of redox metabolism in drug-tolerant persister cells is a vulnerability to prevent relapse in pancreatic cancer

doi: 10.1038/s41389-025-00591-0

Figure Lengend Snippet: A Schematic of experimental protocol. Orthotopic syngeneic allografts were generated by intraperitoneal injection of one million of the murine KPCluc2 cells into 5-week-old female C57BL/6 immunocompetent mice. Tumoral growth was followed by bioluminescence using a Photon Imager device (Biospace Lab). Twelve days post-grafting, tumor-bearing mice were randomized to two treatment cohorts, vehicle control (PBS) and gemcitabine during one month. Mice were sacrificed according to the ethical limit point . B , C Longitudinal in vivo bioluminescence imaging analysis of KPCluc2 cell-grafted mice, untreated (Control) or treated with gemcitabine starting 12 days after implantation. B Bioluminescence images. C Bioluminescent signal quantification in which each line, solid or dotted, represents one mouse (RLU = relative light unit). Tumors in mice 2, 3, 4, 5, 7, and 8 responded to gemcitabine treatment but did not completely regress (Gem group); their numbers are shown in pink. In contrast, tumors in mice 1, 6, 9, 10, 11, and 12 regressed completely as evidenced by the absence or very low level of bioluminescence at at least one stage of treatment effect analysis (Gem-CR group); their numbers are shown in purple. The bioluminescent signal is expressed in photons per second per square centimeter per steradian (ph/s/cm 2 /sr). D Mitochondrial mass, MMP, Mitochondrial O2.- and total ROS level were measured in tumors as in Fig. . Median fluorescence intensity (MFI) is shown relative to unlabeled cells. Each dot corresponds to one mouse, and the bars show the mean ± SD. E Total ATP level was measured using the cell viability assay (Cell-Titer Glo Kit). Unpaired Student’s T -test was used for statistical analyzes comparing each group with that of the untreated. *, **, *** and **** correspond to p < 0.05, 0.01, 0.001, and 0.0001, respectively; ns non-significant difference.

Article Snippet: One million KPCluc2 cells were IP injected into 5- to 6-week-old C57BL/6 female mice (immunocompetent strain, SOPF health status, Charles River, France).

Techniques: Generated, Injection, Control, In Vivo, Imaging, Fluorescence, Viability Assay